jamtJournal of Advanced Materials and TechnologiesJournal of Advanced Materials and Technologies2782-21922782-2206ФГБОУ ВО «ТГТУ»10.17277/jamt-2025-10-03-247-258jamt-62Research ArticleБиологические материалы, наномедицина и новые технологии для клинического и медицинского примененияBiological materials; nanomedicine, and novel technologies for clinical and medical applicationsИсследование антибактериальных свойств экзо- и эндометаболитов микроводорослей Chlorella sorokiniana и цианобактерий Anabaena sphaerica IPPAS B-404Antibacterial properties of exo- and endometabolites of microalgae Chlorella sorokiniana and cyanobacteria Anabaena sphaerica IPPAS B-404https://orcid.org/0000-0001-8642-8558УстинскаяЯ. В.UstinskayaYa. V.

Устинская Яна Витальевна, кандидат технических наук, доцент

ул. Советская, 106/5, пом. 2, Тамбов, 392000

Yana V. Ustinskaya, Cand. Sc. (Eng.), Associate Professor

Bld. 2, 106/5, Sovetskaya St., Tambov, 392000

ustinskaya.yana@yandex.ruhttps://orcid.org/0000-0003-2110-1305ТемновМ. С.TemnovM. S.

Темнов Михаил Сергеевич, кандидат технических наук, доцент

ул. Советская, 106/5, пом. 2, Тамбов, 392000

Mikhail S. Temnov, Cand. Sc. (Eng.), Associate Professor

Bld. 2, 106/5, Sovetskaya St., Tambov, 392000

temnov.mihail@mail.ruhttps://orcid.org/0000-0001-5774-8915МеронюкК. И.MeronyukK. I.

Меронюк Кирилл Иванович, аспирант

ул. Советская, 106/5, пом. 2, Тамбов, 392000

Kirill I. Meronyuk, PhD Student

Bld. 2, 106/5, Sovetskaya St., Tambov, 392000

mirych.87@yandex.ruhttps://orcid.org/0000-0001-5022-0085БрянкинаА. К.BryankinaA. K.

Брянкина Александра Константиновна, аспирант

ул. Советская, 106/5, пом. 2, Тамбов, 392000

Aleksandra K. Bryankina, PhD Student

Bld. 2, 106/5, Sovetskaya St., Tambov, 392000

alex.tambov.ru@mail.ruhttps://orcid.org/0000-0002-7571-3748ДворецкийС. И.DvoretskyS. I.

Дворецкий Станислав Иванович, доктор технических наук, профессор

ул. Советская, 106/5, пом. 2, Тамбов, 392000

Stanislav I. Dvoretsky, D. Sc. (Eng.), Professor

Bld. 2, 106/5, Sovetskaya St., Tambov, 392000

sdvoretsky@mail.tstu.ruhttps://orcid.org/0000-0002-4352-810XДворецкийД. С.DvoretskyD. S.

Дворецкий Дмитрий Станиславович, доктор технических наук, профессор, заведующий кафедрой

ул. Советская, 106/5, пом. 2, Тамбов, 392000

Dmitry S. Dvoretsky, D. Sc. (Eng.), Professor, Head of the Department

Bld. 2, 106/5, Sovetskaya St., Tambov, 392000

dvoretsky@yahoo.comТамбовский государственный технический университетРоссияTambov State Technical UniversityRussian Federation202515022026103247258Copyright © Устинская Я.В., Темнов М.С., Меронюк К.И., Брянкина А.К., Дворецкий С.И., Дворецкий Д.С., 20262026Устинская Я.В., Темнов М.С., Меронюк К.И., Брянкина А.К., Дворецкий С.И., Дворецкий Д.С.Ustinskaya Y.V., Temnov M.S., Meronyuk K.I., Bryankina A.K., Dvoretsky S.I., Dvoretsky D.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://jamt.elpub.ru/jour/article/view/62

Целью работы является исследование антибактериальных свойств биологически активных экзо- и эндометаболитов микроводоросли IPPAS C-1 Chlorella sorokiniana Shihira & R. W. Krauss и цианобактерии IPPAS B-404 Anabaena sphaerica Born. et Flah., как при уровне фотосинтетически активной радиации 100 мкмоль фотонов/(м2⋅с), так и в темноте. Установлена зависимость количественного состава внутриклеточных метаболитов (водорастворимых белков и неполярных липидов) Chlorella sorokiniana и Anabaena sphaerica IPPAS B-404 от условий культивирования, а именно от уровня фотосинтетически активной радиации и температуры. Выявлено, что условия культивирования штаммов влияют на эффективность антибактериального действия данных метаболитов. Экспериментально определены значения минимальных ингибирующих концентраций неполярных веществ липидной природы и водорастворимых пептидных фракций в отношении грамположительной бактериальной культуры, выделенной из воздуха. Продемонстрирована антибактериальная активность неполярных липидов, экстрагированных из культуральной жидкости цианобактерии Anabaena sphaerica IPPAS B-404, в отношении используемых тест-культур бактерий: Bacillus subtilis B-3395, Enterobacter asburiae B-1048, Enterococcus durans B-603 в темноте, и на свету при уровне фотосинтетически активной радиации 100 мкмоль фотонов/(м2⋅с).

This study investigated the antibacterial properties of the biologically active exo- and endometabolites of the microalgae Chlorella sorokiniana Shihira & R.W. Krauss IPPAS C-1 and the cyanobacteria Anabaena sphaerica Born. et Flah. IPPAS B-404, under photosynthetically active radiation of 100 μmoL photons⋅(m2⋅s)–1 and in darkness. The dependence of the quantitative composition of intracellular metabolites (water-soluble proteins and non-polar lipids) of Chlorella sorokiniana and Anabaena sphaerica IPPAS B-404 on the cultivation conditions, namely the level of photosynthetically active radiation and temperature, was determined. The results revealed that the cultivation conditions influence the antibacterial efficiency of these metabolites. The values of minimum inhibitory concentrations of non-polar substances of lipid nature and water-soluble peptide fractions against Gram-positive bacterial culture isolated from air were determined experimentally. The antibacterial activity of non-polar lipids extracted from the culture fluid of the cyanobacterium Anabaena sphaerica IPPAS B-404 was demonstrated against the test bacterial cultures used: Bacillus subtilis B-3395, Enterobacter asburiae B-1048, Enterococcus durans B-603, both in darkness and under 100 μmoL photons⋅(m2⋅s)–1 of photosynthetically active radiation.

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The authors declare that there are no conflicts of interest present.