Исследование антибактериальных свойств экзо- и эндометаболитов микроводорослей Chlorella sorokiniana и цианобактерий Anabaena sphaerica IPPAS B-404

Целью работы является исследование антибактериальных свойств биологически активных экзо- и эндометаболитов микроводоросли IPPAS C-1 Chlorella sorokiniana Shihira & R. W. Krauss и цианобактерии IPPAS B-404 Anabaena sphaerica Born. et Flah., как при уровне фотосинтетически активной радиации 100 мкмоль фотонов/(м²⋅с), так и в темноте. Установлена зависимость количественного состава внутриклеточных метаболитов (водорастворимых белков и неполярных липидов) Chlorella sorokiniana и Anabaena sphaerica IPPAS B-404 от условий культивирования, а именно от уровня фотосинтетически активной радиации и температуры. Выявлено, что условия культивирования штаммов влияют на эффективность антибактериального действия данных метаболитов. Экспериментально определены значения минимальных ингибирующих концентраций неполярных веществ липидной природы и водорастворимых пептидных фракций в отношении грамположительной бактериальной культуры, выделенной из воздуха. Продемонстрирована антибактериальная активность неполярных липидов, экстрагированных из культуральной жидкости цианобактерии Anabaena sphaerica IPPAS B-404, в отношении используемых тест-культур бактерий: Bacillus subtilis B-3395, Enterobacter asburiae B-1048, Enterococcus durans B-603 в темноте, и на свету при уровне фотосинтетически активной радиации 100 мкмоль фотонов/(м²⋅с).

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